Formation of HETE-EAs and dihydroxy derivatives in mouse kidney tissue and analysis by high-performance liquid chromatography tandem mass spectrometry
Laboratory studyOther animals
- Design
- Laboratory study
- Subjects
- C57BL/6J and FAAH knockout mice; numbers not stated in the abstract
- Dose used in the study
- No palmitoylethanolamide was given; a single intravenous bolus of anandamide 20 mg/kg
- Duration
- Not stated in the abstract
- What was measured
- An extraction and HPLC-MS/MS method for anandamide, 2-AG, their oxidised metabolites, oleoylethanolamide and palmitoylethanolamide in kidney, and formation of DiHETE-EAs in vivo.
What the authors reported
The authors synthesised reference DiHETE-EA metabolites using mouse liver cytosol and present a solid phase extraction and HPLC-MS/MS method that quantifies anandamide, 2-AG, their metabolites, oleoylethanolamide and palmitoylethanolamide, and detects DiHETE-EAs formed in kidney after anandamide injection.
Limits of this study
An analytical methods paper in mice; palmitoylethanolamide was one measured analyte and no PEA was given. It cannot show effect in people. The authors declare no conflict of interest.
Source
PubMed 31437772 · doi:10.1016/j.jchromb.2019.121748
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.