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α-Acylamino-β-lactone N-Acylethanolamine-hydrolyzing Acid Amidase Inhibitors Encapsulated in PLGA Nanoparticles: Improvement of the Physical Stability and Protection of Human Cells from Hydrogen Peroxide-Induced Oxidative Stress

Agnese Gagliardi, Roberto Molinaro, Massimo Fresta, Andrea Duranti, Donato Cosco. Antioxidants (Basel). 2022 Mar 31;11(4):686.

Laboratory studyHumansWith URB866 (a NAAA inhibitor that raises the body's own palmitoylethanolamide), loaded in PLGA nanoparticles; PEA itself was not given

This study tested palmitoylethanolamide together with URB866 (a NAAA inhibitor that raises the body's own palmitoylethanolamide), loaded in PLGA nanoparticles; PEA itself was not given. Its results cannot be assigned to palmitoylethanolamide alone. More combination studies.

Design
Laboratory study
Subjects
Various human cell lines exposed to hydrogen peroxide
Dose used in the study
URB866 at 25 micromolar in cell culture
Duration
Not stated in the abstract
What was measured
Size, stability, drug retention and photostability of URB866-loaded nanoparticles; antioxidant protection of cells against hydrogen peroxide.

What the authors reported

The nanoparticles had a single-size population, were stable over time and temperature, retained the compound well and improved its photostability compared with the free molecule. At 25 micromolar they showed a better antioxidant profile on several cell lines.

Limits of this study

A formulation and cell culture study; it cannot show effect in people. Palmitoylethanolamide was not tested; the compound blocks the enzyme that breaks PEA down. The authors declare no conflict of interest.

Source

PubMed 35453371 · doi:10.3390/antiox11040686

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.