Determination of endocannabinoids and endocannabinoid-like substances in human K3EDTA plasma - LC-MS/MS method validation and pre-analytical characteristics
Laboratory studyHumans
- Design
- Laboratory study
- Subjects
- Human K3EDTA plasma and whole blood samples; numbers not stated in the abstract
- Dose used in the study
- Not applicable; no palmitoylethanolamide was given
- Duration
- Not stated in the abstract
- What was measured
- Validation of an LC-MS/MS method for anandamide, oleoylethanolamide, palmitoylethanolamide, 1-AG and 2-AG in plasma, and how sample storage time and temperature change measured levels.
What the authors reported
This laboratory study found:
- The method was validated and separated key isomers.
- During storage, 1-AG and 2-AG rose in plasma, and anandamide, oleoylethanolamide and palmitoylethanolamide rose in whole blood, depending on time and temperature.
- The increases could not be prevented.
- The authors question whether K3EDTA plasma suits these measurements and call summing 1-AG and 2-AG highly questionable.
Limits of this study
An analytical methods study, not a treatment study. Its relevance is that measured palmitoylethanolamide levels in blood depend on sample handling. Funding and conflicts not stated in the abstract.
Source
PubMed 31357310 · doi:10.1016/j.talanta.2019.06.004
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.