Activation of cannabinoid type 1 receptor (CB1) modulates oligodendroglial process branching complexity in rat hippocampal cultures stimulated by olfactory ensheathing glia-conditioned medium

Yolanda Paes-Colli, Priscila M P Trindade, Louise C Vitorino, Fabiana Piscitelli, Fabio Arturo Iannotti, Raquel M P Campos, Alinny R Isaac, Andrey Fabiano Lourenço de Aguiar, Silvana Allodi, Fernando G de Mello, Marcelo Einicker-Lamas, Raphael de Siqueira-Santos, Vincenzo Di Marzo, Bakhos A Tannous, Litia A Carvalho, Ricardo A De Melo Reis, Luzia S Sampaio. Front Cell Neurosci. 2023 Apr 17:17:1134130.

Laboratory studyOther animals

Design

Laboratory study

Subjects

Rat olfactory ensheathing glia cultures and hippocampal mixed cell cultures

Dose used in the study

No palmitoylethanolamide was added; PEA was measured in conditioned medium. Cultures were treated with URB597 or JZL184 at 10^-9 M and AM251 at 10^-6 M

Duration

Not stated in the abstract

What was measured

Endocannabinoid system markers in olfactory ensheathing glia; anandamide, 2-AG, PEA and OEA in conditioned medium; oligodendrocyte process branching by Sholl analysis; Akt and ERK phosphorylation

What the authors reported

Olfactory ensheathing glia expressed CB1, FAAH and MAGL, and released anandamide, 2-AG, PEA and OEA. Their conditioned medium increased oligodendrocyte branching, an effect blocked by a CB1 antagonist, while medium enriched with OEA or 2-AG did not change branching in premyelinating cells and reduced it in mature cells. Akt and ERK phosphorylation did not change.

Limits of this study

A rat cell-culture study; it cannot show effect in people or animals treated in practice. PEA was measured, not tested. The authors declare no commercial or financial conflicts.

Source

PubMed 37138770 · doi:10.3389/fncel.2023.1134130

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.