Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells

Sara Standoli, Cinzia Rapino, Camilla Di Meo, Agnes Rudowski, Nicole Kämpfer-Kolb, Luisa Michelle Volk, Dominique Thomas, Sandra Trautmann, Yannick Schreiber, Dagmar Meyer Zu Heringdorf, Mauro Maccarrone. Int J Mol Sci. 2023 May 9;24(10):8508.

Laboratory studyOther animals

Design

Laboratory study

Subjects

BV2 mouse microglia cell line challenged with LPS

Dose used in the study

URB597 (FAAH inhibitor) or JWH133 (CB2 agonist); palmitoylethanolamide itself was not added

Duration

Not stated in the abstract

What was measured

TNF-alpha and IL-1beta production, levels of anandamide and related lipids including PEA, and transcription of the sphingosine kinases SphK1 and SphK2.

What the authors reported

This laboratory study found:

• URB597 prevented LPS-induced TNF-alpha and IL-1beta and caused accumulation of anandamide, PEA and other ethanolamides.
• JWH133 mimicked the effect of URB597.
• LPS induced SphK1 and SphK2, and inhibiting either kinase strongly reduced cytokine production.
• Both URB597 and JWH133 prevented LPS-induced SphK1 and SphK2 transcription.

Limits of this study

A mouse cell line study; it cannot show effect in people or animals. PEA rose as one of several lipids after FAAH inhibition and was not tested alone. The authors declare no conflict of interest.

Source

PubMed 37239854 · doi:10.3390/ijms24108508

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.