Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane
Laboratory studyHumans
- Design
- Laboratory study
- Subjects
- Human amniotic membrane allografts: fresh, decontaminated, cryopreserved and freeze-dried
- Dose used in the study
- Not applicable; palmitoylethanolamide was measured in tissue, not given
- Duration
- Cryopreserved storage up to the usual 3 to 5 year expiry; freeze-dried after one year
- What was measured
- Concentrations of PEA, OEA and anandamide by UHPLC-MS/MS and PPAR-alpha receptor staining
What the authors reported
The study found:
- PEA, OEA and AEA rose after decontamination.
- Cryopreservation lowered PEA compared with decontaminated tissue but not OEA or AEA, and all three stayed higher than in fresh tissue.
- Freeze-dried membrane matched decontaminated tissue.
- PPAR-alpha staining was visible in all specimens.
- The authors conclude NAEs persist in storage at levels they consider sufficient for analgesic effect.
Limits of this study
A tissue measurement study; it did not test pain relief in people. The claim of a strong analgesic effect is inferred from concentrations, not measured. The authors declare no conflict of interest.
Source
PubMed 37370671 · doi:10.3390/bioengineering10060740
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.