Role of pannexin-1 in the cellular uptake, release and hydrolysis of anandamide by T84 colon cancer cells
Laboratory studyHumans
- Design
- Laboratory study
- Subjects
- Human T84 colon cancer cells, PC3 prostate cancer cells and SH-SY5Y neuroblastoma cells
- Dose used in the study
- Palmitoylethanolamide applied to cells for uptake assays; concentration not stated in the abstract
- Duration
- Uptake and release measured over minutes
- What was measured
- Whether pannexin-1 inhibitors (carbenoxolone, mefloquine) and substrate SR101 change uptake, hydrolysis and release of anandamide, 2-AG and palmitoylethanolamide.
What the authors reported
This lab study found:
- T84 cells expressed pannexin-1 and released ATP in a way the inhibitors reduced.
- The inhibitors did not change uptake or hydrolysis of anandamide, and uptake of 2-AG and palmitoylethanolamide was likewise unaffected.
- Release of tritium from labelled cells was increased rather than blocked.
- Anandamide uptake in PC3 cells was also not inhibited.
- Pannexin-1 does not appear to govern uptake in these cells.
Limits of this study
A cell-culture study; it cannot show effect in people. Palmitoylethanolamide was a secondary test compound. The authors declare no competing interests.
Source
PubMed 31110238 · doi:10.1038/s41598-019-44057-x
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.