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Endocannabinoid and N-acylethanolamide levels in rat brain and spinal cord following systemic dipyrone and paracetamol administration

Ruhan Deniz Topuz, Ozgur Gunduz, Cetin Hakan Karadag, Dikmen Dokmeci, Ahmet Ulugol. Can J Physiol Pharmacol. 2019 Nov;97(11):1035-1041.

Laboratory studyOther animals

Design
Laboratory study
Subjects
Wistar rats; numbers not stated in the abstract
Dose used in the study
No palmitoylethanolamide was given; dipyrone 150, 300 or 600 mg/kg or paracetamol 30, 100 or 300 mg/kg by intraperitoneal injection
Duration
Nociception tested at 1, 5 and 12 hours after injection
What was measured
Tail-flick and hot-plate responses; anandamide, 2-AG, palmitoylethanolamide and oleoylethanolamide in periaqueductal grey, rostral ventromedial medulla and spinal cord by LC-MS/MS.

What the authors reported

This laboratory study found:

  • Both drugs were antinociceptive.
  • Paracetamol raised 2-AG in the PAG and RVM at 12 hours, but dipyrone did not.
  • Both analgesics lowered anandamide in the RVM and spinal cord, with similar decreases in palmitoylethanolamide and oleoylethanolamide in those regions.
  • The authors describe the overall pattern of effects as complicated.

Limits of this study

A rat study measuring endogenous levels after common analgesics; no PEA was given and it cannot show effect in people. Funding and conflicts not stated in the abstract.

Source

PubMed 31283890 · doi:10.1139/cjpp-2019-0015

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.