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Genetic Blockade of NAAA Cell-specifically Regulates Fatty Acid Ethanolamides (FAEs) Metabolism and Inflammatory Responses

Xiaohua Xie, Yitian Li, Sennan Xu, Pan Zhou, Longhe Yang, Yaping Xu, Yan Qiu, Yungang Yang, Yuhang Li. Front Pharmacol. 2022 Jan 7:12:817603.

Laboratory studyOther animalsWith NAAA gene deletion (CRISPR-Cas9) or the NAAA inhibitor F96, which raise the body's own palmitoylethanolamide; PEA itself was not given

This study tested palmitoylethanolamide together with NAAA gene deletion (CRISPR-Cas9) or the NAAA inhibitor F96, which raise the body's own palmitoylethanolamide; PEA itself was not given. Its results cannot be assigned to palmitoylethanolamide alone. More combination studies.

Design
Laboratory study
Subjects
NAAA-deficient and wild-type mice, including bone marrow chimeras; numbers not stated in the abstract
Dose used in the study
Not stated in the abstract
Duration
Not stated in the abstract
What was measured
PEA and AEA levels in bone marrow, macrophages and lungs; inflammation in LPS-induced acute lung injury; pain in carrageenan hyperalgesia and sciatic nerve injury models

What the authors reported

The study found:

  • NAAA deletion raised PEA and AEA in bone marrow and macrophages.
  • Unexpectedly, gene deletion gave only moderate anti-inflammatory effects in lung injury and poor pain relief, whereas the inhibitor F96 gave marked anti-inflammation and analgesia.
  • Chimera experiments showed deleting NAAA in bone marrow-derived cells produced strong effects, while absence in non-bone-marrow cells did not.

Limits of this study

A mouse study; it cannot show effect in people. No palmitoylethanolamide was given. The authors declare no commercial or financial relationships.

Source

PubMed 35069223 · doi:10.3389/fphar.2021.817603

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.